LOCUS       40924_23919       12207 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB791 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Gateway Binary Vectors with Organelle-Targeted Fluorescent Proteins 
            for Highly Sensitive Reporter Assay in Gene Expression Analysis of 
            Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12207)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane 
            University, Interdisciplinary Center for Science Research; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12207)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12207)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane University, 
            Interdisciplinary Center for Science Research; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2103..2114)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     CDS             2236..2946
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      2971..3223
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3257..3273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3281..3297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3305..3335)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3350..3371)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3407..3659)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3697..4992)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BBG75682.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3697..4992)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5044..5223)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5729..5753)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6274..7062
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7311..7899
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8085..8225)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8569..8763)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8832..9902)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10334..10960)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggca atggctgttt tccgtcgcga agggaggcgt
     2101 ctcctccctt caatcgccgc tcgcccaatc gctgctatcc gatctcctct ctcttctgac
     2161 caggaggaag gacttcttgg agttcgatct atctcaactc aagtggtgcg taaccgcatg
     2221 aagagtgtta agaacatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg
     2281 aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa
     2341 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     2401 cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcagaac cttcatcaac
     2461 cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag
     2521 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     2581 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct
     2641 gtgatgcaga agaaaacact cggctgggag gccaacaccg agatgctgta ccccgctgac
     2701 ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg tgggcggggg ccacctgatc
     2761 tgcaacttca agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
     2821 gtctactatg tggaccacag actggaaaga atcaaggagg ccgacaaaga gacctacgtc
     2881 gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa
     2941 cttaattgag cttagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     3001 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     3061 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     3121 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     3181 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa
     3241 ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3301 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3361 aactcacatt aggctgaatt aggcgcgcct atttctatta attcccgatc tagtaacata
     3421 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     3481 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     3541 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     3601 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg
     3661 gggaaattcg cctcgagtga cttctctgat tgcagactat ttataccaac cagcaagaaa
     3721 gtgtcgaagt atgaagcaga aggcgcaggc tagagcctgg aaaacgagaa gatggatgca
     3781 aagagacttt tctgatttag gaccaaacaa cctcaagtaa acattgacga gtgtcccatc
     3841 ttttgtgccg gtcagtaatc cggtagcagg atcatacctt gggagacgat ggcgcggaca
     3901 tttcacaatc ccagcaagct gaggaagcga taatagaagg ttcagaactt gaggaagaaa
     3961 gaagatgagg agggtttcac tgaagtgacc cagaatacca acaactgcca tagtcattcc
     4021 ggcgaagact gtgtaagtgt ctccaacaaa aactgaagaa ggataccaat tgtaagcaag
     4081 cattgccaag gatgtggcca tcagcggttg agtaagaaat atcgagaagg catgagcttg
     4141 gtgatactcg ggatcaacag atgctccgat ttgcataacg ttgtgtatca ggatagcagc
     4201 ggcaataaca actgtttgac cgatttcaag gccattcaga ccagcgtgga tgttaataga
     4261 gtttgtacaa aagacagcaa gtagccccat atataactta tatatccttc ctagattaag
     4321 cacttccaag ccaatataag caacaagagg ttttggtata acaatagttg tatgaccagc
     4381 ataagccatc aaaagtggaa gggtagcaaa agatggcagg acaagtttta ctctccaagg
     4441 cacatcaagg acatcatcta caaatccaag caaaatcatg aagcaaatag atgctagtgc
     4501 tgcattatac tccacaagcc aattagaatc ttcagtgaag ttaaaatact gaaatattat
     4561 cgccacgatc aaaaagacaa taccaacaac aatacccaat gactctggca ctttaatatc
     4621 tccttgaggc gtgcctcttt tgttgatatc aaaaccaaac atgttacgtc tcagaacgta
     4681 tctggcagta acaggaatca ttttgagagt gacgaaaaac ccaaccaagc taagccccgc
     4741 gttaattaga atcgatcgct tgagttcatt atcaaccttg tagtgaaaac agagaaggta
     4801 tagatagagc gaacagagaa gagtagagat cacgaagatc actcctaatt tcggcggcgc
     4861 gagacgaaat tcctcaccgg aagcagaaac cgtctttcgg gtcatcttct gctccgatgg
     4921 cgctgagttg ggttccgacg ggtccggttt gttgggaatt gagatcgatg aagctctctt
     4981 acgagctgcc atatcttatt gccccccggg atcagatctg gattgagagt gaatatgaga
     5041 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat
     5101 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt
     5161 atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg
     5221 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg
     5281 actcccttaa ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa
     5341 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     5401 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     5461 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     5521 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
     5581 cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaagctgatc cgtcgagatt
     5641 aatagaaata aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg
     5701 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     5761 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     5821 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     5881 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     5941 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca
     6001 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     6061 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     6121 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     6181 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     6241 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
     6301 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     6361 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     6421 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     6481 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     6541 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     6601 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     6661 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     6721 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     6781 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     6841 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     6901 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     6961 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     7021 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
     7081 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     7141 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     7201 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag
     7261 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7321 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7381 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7441 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     7501 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7561 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7621 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7681 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7741 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7801 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7861 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7921 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7981 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8041 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     8101 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     8161 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     8221 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8281 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8341 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     8401 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     8461 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     8521 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     8581 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     8641 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     8701 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     8761 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     8821 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     8881 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     8941 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     9001 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     9061 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     9121 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     9181 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     9241 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     9301 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     9361 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     9421 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     9481 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     9541 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     9601 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     9661 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     9721 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     9781 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     9841 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     9901 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     9961 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
    10021 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
    10081 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
    10141 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
    10201 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
    10261 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
    10321 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
    10381 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
    10441 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
    10501 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
    10561 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    10621 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    10681 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    10741 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    10801 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    10861 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    10921 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    10981 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    11041 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    11101 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    11161 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    11221 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    11281 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    11341 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    11401 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    11461 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    11521 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    11581 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    11641 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    11701 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    11761 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    11821 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    11881 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    11941 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    12001 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    12061 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    12121 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    12181 catacaaatg gacgaacgga taaacct
//
